In order to improve the quality and efficiency of railway crew rostering plan arrangement, the problem of crew rostering plan arrangement was abstracted as a Multi-Traveling Salesman Problem (MTSP) with single base and considering mid-way rest, a single-circulation crew rostering plan mathematical model aiming at the smallest rostering period and the most balanced distributed redundant connection time between crew routings was established, and a new amended heuristic ant colony optimization algorithm was proposed aiming at the model. Firstly, a solution space satisfying the spatial-temporal constraints was constructed and the pheromone concentration was set for the crew routing nodes and the continued paths respectively. Then, the amended heuristic information was adopted to make the ants start at the crew routing order and go through all the crew routings. Finally, the optimal crew rostering plan was selected from the different crew rostering schemes. The proposed model and algorithm were tested on the data of the intercity railway from Guangzhou to Shenzhen. The comparison results with the plan arranged by particle swarm optimization show that under the same model conditions, the crew rostering plan arranged by amended heuristic ant colony optimization algorithm has the average monthly man-hour reduced by 8.5%, the rostering period decreased by 9.4%, and the crew overwork rate of 0. The designed model and algorithm can compress the crew rostering cycle, reduce the crew cost, balance the workload, and avoid the overwork of crew.